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    Benchmark Scientific d1036 superose
    D1036 Superose, supplied by Benchmark Scientific, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/d1036+superose/pm36514310-190-226-222
    Average 96 stars, based on 1 article reviews
    d1036 superose - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Variant Assay:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Software:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Maestro Software:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Real-time Polymerase Chain Reaction:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Flow Cytometry:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Microscopy:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Spectrophotometry:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Luciferase:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..

    Isolation:

    Article Title: Molecular basis for antiviral activity of two pediatric neutralizing antibodies targeting SARS-CoV-2 Spike RBD.
    Article Snippet: The Jackson Laboratory Recombinant DNA pcDNA3.1-SARS-CoV-2 RBD Beaudoin-Bussieres et al.9 N/A pcDNA3.1-SARS-CoV-2 RBD E484K Gong et al.10 N/A pNL4.3 R-E- Luc NIH AIDS Reagent Program Cat # 3418 pIRES2-eGFP vector Clontech Cat # 6029-1 pCG1-SARS-CoV-2 Spike WT (Wuhan-Hu-1) Hoffmann et al., 202011 N/A pCG1-SARS-CoV-2 Spike D614G Beaudoin-Bussieres et al.9 N/A pCG1-SARS-CoV-2 Spike B.1.429 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.1.7 variant Tauzin et al.12 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.351 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike P.1 variant Li et al.3 N/A pcDNA3.1-SARS-CoV-2 Spike B.1.526 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.1 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.617.2 variant Li et al.3 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.1) variant Chatterjee et al.13 N/A pCAGGS-SARS-CoV-2 Spike B.1.1.529 (BA.2) variant Ding et al.14 N/A pCG1-SARS-CoV-2 Spike D614G RBM mutants panel Li et al.3 N/A pLTR-Tat Finzi et al.15 N/A pCMV-S: pCMV3-SARS-CoV-2 Spike (S1+S2)-long Sino Biological Cat # VG40589-UT pACP-tag(m)-2 ACE2-Fc fusion protein Anand et al.16 N/A pTRIOZ-hIgG1 InvivoGen N/A pTRIOZ-hIgG1-EH3 heavy chain This paper N/A pTRIOZ-hIgG1-EH3 light chain This paper N/A pTRIOZ-hIgG1-EH8 heavy chain This paper N/A pTRIOZ-hIgG1-EH8 light chain This paper N/A pACP-SARS-CoV-2 RBDmini(329-527) Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) Chen et al.17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.7 variant Chen et al.17 N/A pACP-SARS-CoV-2 RBDmini (329-527) B.1.351 variant Chen et al. 17 N/A pACP-SARS-CoV-2 RBD (319-537) B.1.429 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.526 variant InvivoGen, this paper N/A (Continued on next page) 20 iScience 26, 105783, January 20, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER pACP-SARS-CoV-2 RBD (319-537) B.1.617.2 variant InvivoGen, this paper N/A pACP-SARS-CoV-2 RBD (319-537) B.1.1.529.1 variant GenScript, this paper N/A Software and algorithms FlowJo v10.5.3 Treestar https://www.flowjo.com/ Graphpad Prism v9.1.0 GraphPad Software https://www.graphpad.com/ CFX Maestro Software (qPCR analyses) Bio-rad Inc N/A Living Image v4.7.3 PerkinElmer N/A Phenix Liebschner et al.53 https://phenix-online.org/ Coot Emsley et al.54 https://www2.mrc-lmb.cam. ac.uk/personal/pemsley/coot/ UCSF ChimeraX Goddard et al.55 https://www.rbvi.ucsf.edu/chimerax/ PyMOL The PyMOL Molecular Graphics System https://www.schrodinger. com/products/pymol MolProbity Williams et al.56 http://molprobity.biochem.duke.edu/ PISA Krissinel and Henrick,19 https://www.ebi.ac.uk/pdbe/pisa/ MATLAB Mathworks https://www.mathworks.com SPARTAN St. Jude Children’s Research Hospital https://www.scottcblanchardlab. com/software BI-Aevaluation software GE Healthcare Cat # BR-1005-97 Others TriStar LB 942 Multimode Microplate Reader and Luminometer BERTHOLD TECHNOLOGIES GmbH & Co. KG N/A BD LSR II Flow Cytometer BD Biosciences N/A MinION sequencer Oxford Nanopore Technologies N/A FLO-MIN106 flowcell Oxford Nanopore Technologies N/A BioAnalyzer 2100 Agilent Technologies N/A Maxisorp Nunc White 96-Well Flat-Bottom Microplate ThermoFisher Scientific Cat # 437796 Prism-based TIRF Microscope Mothes Lab N/A ORCA-Flash4.0 V3 sCMOS camera Hamamatsu N/A C1000 Touch thermal cycler Bio-Rad N/A CFX Connect Real-Time PCR Detection System Bio-Rad N/A Nanodrop Spectrophotometer ND-1000 ThermoFisher Scientific N/A 96-well white plates for luciferase assays Costar Cat # 3917 XIC-3 animal isolation chamber PerkinElmer Cat # 123997 IVIS Spectrum PerkinElmer Cat # 124262 RAS-4 Rodent Gas Anesthesia System PerkinElmer Cat # CLS146737 BeadBug 6 homogenizer Benchmark Scientific Cat # D1036 Superose 6 10/300 GL GE Healthcare Cat # 17517201 Hiload 26/600 Superdex 200 pg GE Healthcare Cat # 28989335 Biacore 3000 Cytiva N/A Protein A sensor chip Cytiva Cat # 29127558 Octet RED96e System ForteBio N/A iScience 26, 105783, January 20, 2023 21 ..



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